Interactions of 5′-Adenosine Diphosphate and l-Threonine with the Quaternary Structure of l-Threonine Dehydratase
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Purification and regulatory properties of the adenosine diphosphate-activated threonine dehydratase.
The ADP-activated threonine dehydratase of Closfridium fefanomorphum has been purified approximately llOO-fold in 20% yield and crystallized. Such preparations are homogeneous when examined by sedimentation, electrophoretic, and immunological techniques. The pure enzyme displays an absorption maximum at 415 rnp in neutral solutions, which is consistent with a Schiff base linkage between pyridox...
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In order to clarify the mechanism of activation of L-threonine deaminase by adenosine diphosphate, the enzyme was purified about 700.fold from sonic extracts of Closlridium tetanomorphum, and kinetic and ADP-binding studies were carried out. Plots of reaction rates against L-threonine concentrations gave a sigmoid curve in the absence of ADP, whereas a hyperbolic curve was obtained in the prese...
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Selim and Greenberg (1, 2) achieved a considerable degree of purification of L-serine dehydratase (L-serine hydro-lyase (deaminating), EC 4.2.1.13) from rat liver and demonstrated that this protein preparation contained the cystathionine-synthesizing activity of the liver (L-serine hydro-lyase (adding L-homocysteine), EC 4.2.1.21). These workers (2) also observed activity of their enzyme prepar...
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Preclinical data indicate that the administration of the amino acid L-threonine increases glycine levels in rat spinal cord. In order to investigate glycinergic mechanisms in spasticity, and other signs of the upper motor syndrome, we gave 4.5 and 6.0 g/day of L-threonine to 18 patients with familial spastic paraparesis (FSP) according to a double-blind, crossover protocol. The response to trea...
متن کاملRat liver L-threonine dehydrogenase.
L-threonine dehydrogenase (E.C.1.1.1.103) catalyzes the NAD+-dependent transformation of Lthreonine to 2-amino 3-0x0-butyric acid, which spontaneously loses CO2 with the final production of aminoacetone ( 1) The enzyme is present in several microorganisms as well as in the liver of vertebrates (2-6). We studied some properties of the rat liver enzyme, showing that the activity was strongly inhi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)42785-3